Amines
REACH
Tris(hydroxymethyl)aminomethane
CAS Number
77-86-1
EC Number
201-064-4
Abbreviation
Tris
What is Tris(hydroxymethyl)aminomethane?
Tris(hydroxymethyl)aminomethane (CAS 77-86-1), commonly known as Tris or Tromethamine, is an organic amine base used in pharmaceutical, cosmetic, and life science manufacturing.
Where is Tris(hydroxymethyl)aminomethane used?
- Pharmaceutical manufacturing: neutralizes acidic drug substances and buffers injectables, ophthalmic solutions, and topical preparations
- Cosmetics: adjusts and stabilizes pH in creams, gels, and emulsions, particularly where carbomer polymers require neutralization
- Life science: Tris buffers, most notably Tris-HCl, serve as the standard medium for DNA/RNA electrophoresis
- It is also essential for protein extraction and cell lysis protocols in laboratory settings.
Commercial specifications
- High-purity molecular biology grades for research
Other Names (Synonyms)
2-amino-2-(hydroxymethyl)propane-1,3-diol|Tromethamine|Tris
What are the physical properties of Tris(hydroxymethyl)aminomethane?
Melting Point 171-172 °C
Boiling Point 219-220 °C @ Press: 10 Torr
Density 1.02835 g/cm³ @ Temp: 25.00 °C
Flash Point 219-220°C/10mm
Appearance White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.
Color white
Form crystalline
Water Solubility 550 g/L (25 ºC)
Vapor Pressure 0.0267 hPa (20 °C)
Refractive Index 1.4170 (estimate)
pH 10.5-12.0(4 m in water, 25 °C)
Log P -2.515 (est)
How is Tris(hydroxymethyl)aminomethane classified and handled safely? Learn More
Warning
GHS07· Irritant
Hazard Statements
H315 Causes skin irritation
H319 Causes serious eye irritation
H335 May cause respiratory irritation
Precautionary Statements
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P264 Wash … thoroughly after handling.
P271 Use only outdoors or in a well-ventilated area.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
Which trade and regulatory codes apply to Tris(hydroxymethyl)aminomethane?
HS Code 29221980
Storage Class 11 - Combustible Solids
WGK (Germany) 2