Thymidine monophosphate

Thymidine monophosphate (CAS 365-07-1), also known as dTMP or 5'-thymidylic acid, is a nucleotide monophosphate used as a biochemical building block in pharmaceutical synthesis and life science research.

It functions as a precursor in nucleotide metabolism pathways and supports DNA replication processes relevant to drug development and diagnostic applications. In pharmaceutical manufacturing, dTMP serves as an intermediate.

It is used in the synthesis of antiviral and anticancer nucleotide analogues, where structural purity directly affects downstream API quality. Biotechnology producers use it in cell culture media formulations.

These formulations supplement nucleotide pools and support proliferation of nucleotide-auxotrophic cell lines. It is also incorporated into enzymatic assay systems and polymerase chain reaction reagents.

Defined nucleotide concentrations are critical to assay reproducibility in these systems. Research-scale synthesis of oligonucleotides and labeled DNA probes represents an additional procurement application.

These applications are vital in molecular diagnostics development. Thymidine monophosphate is supplied as a white to off-white crystalline powder, typically as the free acid or disodium salt form.

Standard grades include research-grade and pharmaceutical-grade material, with purity specifications commonly at ≥98% or ≥99% by HPLC. Packaging ranges from milligram quantities to kilogram lots.

These options support both laboratory use and process-scale procurement. The material meets rigorous industry requirements for consistency and performance in sensitive biochemical applications.