1-hydroxybenzotriazole

1-Hydroxybenzotriazole (CAS 2592-95-2), commonly known as HOBt, is a coupling reagent and catalyst used in peptide synthesis and pharmaceutical manufacturing.

Where is 1-hydroxybenzotriazole used?

• Peptide synthesis: forms active esters that drive efficient coupling of amino acids in solid-phase and solution-phase routes
• Pharmaceutical manufacturing: used with carbodiimide reagents such as EDC or DCC to improve coupling efficiency
• API production: suppresses racemization and activates carboxyl groups during amide bond formation
• Drug discovery: supports production of peptide libraries
• Contract research organizations rely on HOBt for small-scale synthesis of peptide-based drug candidates.

Good to know: The hydrated form is preferred for safer handling in bulk quantities. Stereochemical integrity is a critical quality parameter that this reagent helps maintain.

Commercial specifications

• Anhydrous form or monohydrate
• Anhydrous and hydrate grades meeting pharmaceutical research specifications
• Laboratory quantities through to multi-kilogram lots