1-hydroxybenzotriazole hydrate

1-Hydroxybenzotriazole hydrate (CAS 80029-43-2), commonly sourced as HOBt hydrate, is a coupling additive used in peptide synthesis and pharmaceutical manufacturing.

Where is 1-hydroxybenzotriazole hydrate used?

• Peptide synthesis: coupling additive with carbodiimide reagents such as DCC or EDC to produce coupling-ready active esters
• Pharmaceutical manufacturing: suppresses racemization and activates carboxyl groups during amide bond formation
• API synthesis: supports high-purity amide intermediates under controlled conditions
• Medicinal chemistry: supports synthesis of peptidomimetics and specialty heterocyclic intermediates
• Contract manufacturers and research organizations use it in the preparation of peptide-based therapeutics.

Good to know: The hydrate form is the standard commercial offering, preferred over the anhydrous grade for its improved handling safety profile in bulk storage and transport.

Commercial specifications

• Pharmaceutical-grade purity requirements, often ≥98% by HPLC
• Research quantities through to multi-kilogram lots