n-hydroxysuccinimide

N-Hydroxysuccinimide (CAS 6066-82-6), commonly abbreviated as NHS, is a key reagent in bioconjugation chemistry used to activate carboxylic acid groups for stable amide bond formation.

Where is n-hydroxysuccinimide used?

• Bioconjugation: coupling of proteins, peptides, antibodies and other biomolecules to surfaces, linkers or drug payloads
• Synthesis of NHS esters as reactive intermediates for antibody-drug conjugate (ADC) manufacturing and targeted therapeutic development
• Diagnostic kits: immobilization of biomolecules onto beads, membranes and microplates
• Peptide synthesis and PEGylation workflows rely on NHS-activated intermediates for controlled, site-specific conjugation under mild aqueous conditions.
• Cosmetic and personal care formulators use NHS-based crosslinking chemistry in hyaluronic acid derivatives and biomaterial scaffolds.

Commercial specifications

• High-purity grades (≥98%, ≥99%) commonly required for GMP bioconjugation and API intermediate applications
• Packaging from laboratory-scale quantities to bulk industrial supply